What is important in this lab?
- What do restriction enzymes do?
- What are sticky ends? Blunt ends?
- What are plasmids? pAMP?
- Why do we leave some restriction enzymes out of the mixes?
- Why do we use gel electrophoresis?
What do the enzymes do?
- They cut at specific sequences. They make circular plasmids into linear strands of DNA!
What happens when you put in more than one enzyme?
- You get multiple cuts which means multiple strands of DNA.
Why do we use gel electrophoresis?
- To determine the size of the fragments after the cuts
- To determine the plasmid map and where in relation to one another the enzymes cut.
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